Protein Crystallization and X-Ray Diffraction Course (pcxrd)
The practical course in crystallization and diffraction is intended to be a precursor to the one semester graduate level macromolecular crystallization special topics course taught by 9-month faculty every other year. In this course, we will cover basic concepts of crystals, symmetry, crystallization, x-ray generation, radiation safety, x-ray diffraction, cryo safety, cryogenic technique, data collection, data processing, Linux o/s, computer graphics, data visualization, and model building. More emphasis will be given to hands-on training and lab work than the full-fledged graduate course.
At the end of this course, you will learn basic concepts in:
- Protein crystallization and crystal symmetry
- Cryogenic safety and cryo-crystallography
- Need for crystals, cryogenic temperatures, and X-Rays
- X-Ray generation and safety
- X-Ray diffraction and data collection
- Linux operating system and running programs
- Computer graphics and model visualization
Session: We will meet for 1 1/2 hours per session and two sessions per week. The session will start with 30 minutes of lecture followed by 45 minutes of lab-work or hands on training which will be followed by 15 minutes of Question and Answer session.
Course: The total course length is expected to last four weeks with two sessions per week. We expect the course will have 4-8 students per session and all lab supplies will be provided to the students.
1. Symmetry, lattices, crystals, and crystallization
2. Crystals and protein crystallization set-up
3. Crystallization observation and optimization
4. X-Rays, radiation safety and X-Ray generation
5. Cryogenics safety and cryo-crystallography
6. X-Ray Diffraction set-up and data collection
7. Linux operating system and computer graphics
8. Data processing and visualization and wrap-up
Sessions high-lighted in bold will involve more hands-on training time and sessions high-lighted in italics will involve more lecture time than indicated in the original description.
- Duration of the program: 4 weeks
- Number of days for lectures: 3
- Number of days for experiments: 5
- Flexible enough for expansion of experiments and data
- Provide experiences in the wet-lab (biochemistry), dry-lab (crystallography), and computer skills (Linux)