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Protein Crystallization and X-Ray Diffraction Course (pcxrd)

PCXRD

GOALS

The practical course in crystallization and diffraction is intended to be a precursor to the one semester graduate level macromolecular crystallization special topics course taught by 9-month faculty every other year. In this course, we will cover basic concepts of crystals, symmetry, crystallization, x-ray generation, radiation safety, x-ray diffraction, cryo safety, cryogenic technique, data collection, data processing, Linux o/s, computer graphics, data visualization, and model building. More emphasis will be given to hands-on training and lab work than the full-fledged graduate course.

At the end of this course, you will learn basic concepts in:

  • Protein crystallization and crystal symmetry
  • Cryogenic safety and cryo-crystallography
  • Need for crystals, cryogenic temperatures, and X-Rays
  • X-Ray generation and safety
  • X-Ray diffraction and data collection
  • Linux operating system and running programs
  • Computer graphics and model visualization
COURSE SCHEDULE
Session: We will meet for 1 1/2 hours per session and two sessions per week. The session will start with 30 minutes of lecture followed by 45 minutes of lab-work or hands on training which will be followed by 15 minutes of Question and Answer session. Course: The total course length is expected to last four weeks with two sessions per week. We expect the course will have 4-8 students per session and all lab supplies will be provided to the students.
SYLLABUS
  • 1. Symmetry, lattices, crystals, and crystallization
  • 2. Crystals and protein crystallization set-up
  • 3. Crystallization observation and optimization
  • 4. X-Rays, radiation safety and X-Ray generation
  • 5. Cryogenics safety and cryo-crystallography
  • 6. X-Ray Diffraction set-up and data collection
  • 7. Linux operating system and computer graphics
  • 8. Data processing and visualization and wrap-up
  • Sessions high-lighted in bold will involve more hands-on training time and sessions high-lighted in italics will involve more lecture time than indicated in the original description.
    • Duration of the program: 4 weeks
    • Number of days for lectures: 3
    • Number of days for experiments: 5
    • Flexible enough for expansion of experiments and data
    • Provide experiences in the wet-lab (biochemistry), dry-lab (crystallography), and computer skills (Linux)
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    © 2003-12 FSU & Thayumanasamy Somasundaram
    www.sb.fsu.edu/~soma/Proj/pcxrd/index.html
    florida state university