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Protein Crystallization and X-Ray Diffraction (pcxrd)

The practical course in crystallization and diffraction is intended to be a precursor to the one semester graduate level macromolecular crystallization special topics course taught by 9-month faculty every other year. In this course, we will cover basic concepts of crystals, symmetry, crystallization, x-ray generation, radiation safety, x-ray diffraction, cryo safety, cryogenic technique, data collection, data processing, Linux o/s, computer graphics, data visualization, and model building. More emphasis will be given to hands-on training and lab work than the full-fledged graduate course.

At the end of this course, you will learn basic concepts in:

  • Protein crystallization and crystal symmetry
  • Cryogenic safety and cryo-crystallography
  • Need for crystals, cryogenic temperatures, and X-Rays
  • X-Ray generation and safety
  • X-Ray diffraction and data collection
  • Linux operating system and running programs
  • Computer graphics and model visualization
MOB Bootcamp (August of every year) | 2012 Course Schedule
  • Duration of the program: 3 weeks
  • Number of days for lectures: 6
  • Number of days for experiments: 5
  • Flexible enough for expansion of experiments and data
  • Provides experience in the wet-lab (biochemistry), instrumentation (crystallography), and computation (Linux and data processing)

The following is the X-Ray Facility schedule for fall 2012. Updated: Tuesday, July 31, 2012.

  • Bootcamp Intro [Wed, Aug 08, 2012]
  • Module 1 A+B [Wed, Aug 08, 2012]
  • Module 2 A+B [Thu, Aug 09, 2012]
  • Module 3 A+B [Tue, Aug 14, 2012]
  • Module 3 A+B [Wed, Aug 15, 2012]
  • Module 5 A+B [Wed, Aug 22, 2012]
  • Module 6 A+B [Thu, Aug 23, 2012]
Course Syllabus [Detailed Syllabus]
  • 1. Symmetry, lattices, crystals, and crystallization
  • 2. Crystals and protein crystallization set-up
  • 3. Crystallization observation and optimization
  • 4. X-Rays, radiation safety and X-Ray generation
  • 5. Cryogenics safety and cryo-crystallography
  • 6. X-Ray Diffraction set-up and data collection
  • 7. Linux operating system and computer graphics
  • 8. Data processing and visualization and wrap-up
Sessions high-lighted in bold will involve more hands-on training time and sessions high-lighted in italics will involve more lecture time.
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